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pL-CRISPR.EFS.tRFP
(Plasmid #57819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 57819 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLKO.005
  • Total vector size (bp) 11701
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SpCas9
  • Species
    S. pyogenes
  • Insert Size (bp)
    4300
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Sp sgRNA scaffold
  • Insert Size (bp)
    75

Gene/Insert 3

  • Gene/Insert name
    EFS
  • Alt name
    short EF1alpha Promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    250
  • Entrez Gene
    EEF1A1 (a.k.a. CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, EF1A1, EF1alpha1, GRAF-1EF, LENG7, PTI1, eEF1A-1)

Gene/Insert 4

  • Gene/Insert name
    P2A-tRFP
  • Alt name
    tagRFP
  • Species
    Synthetic
  • Insert Size (bp)
    750

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use BsmBI sites for sgRNA cloning. Note that this plasmid does NOT contain the 1.9kb stuffer from the pLKO.005 vector backbone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pL-CRISPR.EFS.tRFP was a gift from Benjamin Ebert (Addgene plasmid # 57819)

  • For your References section:

    Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey ME, Thielke A, Aster JC, Regev A, Ebert BL. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. 10.1038/nbt.2951 PubMed 24952903
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