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Addgene

Dendra2-Actin-C-18
(Plasmid #57701)

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Item Catalog # Description Quantity Price (USD)
Plasmid 57701 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    Dendra2-C1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Actin
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001101.3
  • Entrez Gene
    ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
  • Promoter CMV
  • Tag / Fusion Protein
    • Dendra2 (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 490 / 553; Emission = 507 / 573

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dendra2-Actin-C-18 was a gift from Michael Davidson (Addgene plasmid # 57701 ; http://n2t.net/addgene:57701 ; RRID:Addgene_57701)

  • For your References section:

    A bright and photostable photoconvertible fluorescent protein. McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL. Nat Methods. 2009 Feb . 6(2):131-3. 10.1038/nmeth.1296 PubMed 19169260
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