tdEos-PMP-N-10
(Plasmid
#57658)
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PurposeLocalization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 581
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 57658 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonetdEos-N1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePMP
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Alt nameNM_018663.1
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SpeciesH. sapiens (human)
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Entrez GenePXMP2 (a.k.a. PMP22)
- Promoter CMV
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Tag
/ Fusion Protein
- tdEos (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 505 / 569; Emission = 516 / 581
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tdEos-PMP-N-10 was a gift from Michael Davidson (Addgene plasmid # 57658 ; http://n2t.net/addgene:57658 ; RRID:Addgene_57658) -
For your References section:
Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms. Huang F, Hartwich TM, Rivera-Molina FE, Lin Y, Duim WC, Long JJ, Uchil PD, Myers JR, Baird MA, Mothes W, Davidson MW, Toomre D, Bewersdorf J. Nat Methods. 2013 Jul;10(7):653-8. doi: 10.1038/nmeth.2488. Epub 2013 May 26. 10.1038/nmeth.2488 PubMed 23708387