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Addgene

mEos3.2-EB3-7
(Plasmid #57454)

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Item Catalog # Description Quantity Price (USD)
Plasmid 57454 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    mEos3.2
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EB3
  • Alt name
    NM_012326.2
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAPRE3 (a.k.a. EB3, EBF3, EBF3-S, RP3)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEos3.2 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 507 / 572; Emission = 516 / 580

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEos3.2-EB3-7 was a gift from Michael Davidson (Addgene plasmid # 57454 ; http://n2t.net/addgene:57454 ; RRID:Addgene_57454)

  • For your References section:

    Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms. Huang F, Hartwich TM, Rivera-Molina FE, Lin Y, Duim WC, Long JJ, Uchil PD, Myers JR, Baird MA, Mothes W, Davidson MW, Toomre D, Bewersdorf J. Nat Methods. 2013 Jul;10(7):653-8. doi: 10.1038/nmeth.2488. Epub 2013 May 26. 10.1038/nmeth.2488 PubMed 23708387
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