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Addgene

mEos2-Farnesyl-5
(Plasmid #57375)

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Item Catalog # Description Quantity Price (USD)
Plasmid 57375 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    mEos2
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Farnesyl
  • Alt name
    NM_001130442.1
  • Species
    H. sapiens (human)
  • Mutation
    encodes final 20 aa of NM_001130442.1
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEos2

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 505 / 569; Emission = 516 / 581

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEos2-Farnesyl-5 was a gift from Michael Davidson & Catherine Galbraith (Addgene plasmid # 57375 ; http://n2t.net/addgene:57375 ; RRID:Addgene_57375)

  • For your References section:

    Changes in single-molecule integrin dynamics linked to local cellular behavior. Jaqaman K, Galbraith JA, Davidson MW, Galbraith CG. Mol Biol Cell. 2016 May 15;27(10):1561-9. doi: 10.1091/mbc.E16-01-0018. Epub 2016 Mar 23. 10.1091/mbc.E16-01-0018 PubMed 27009207
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