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Addgene

mEos2-Alpha-Actinin-C-14
(Plasmid #57347)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 57347 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    mEos2-C1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Alpha-Actinin
  • Alt name
    NM_001102.3
  • Species
    H. sapiens (human)
  • Entrez Gene
    ACTN1 (a.k.a. BDPLT15)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEos2 (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 505 / 569; Emission = 516 / 581

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEos2-Alpha-Actinin-C-14 was a gift from Michael Davidson (Addgene plasmid # 57347 ; http://n2t.net/addgene:57347 ; RRID:Addgene_57347)
  • For your References section:

    Nanoscale architecture of integrin-based cell adhesions. Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess HF, Waterman CM. Nature. 2010 Nov 25;468(7323):580-4. doi: 10.1038/nature09621. 10.1038/nature09621 PubMed 21107430