Dronpa-Vinculin-23
(Plasmid
#57308)
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PurposeLocalization: Focal Adhesions, Excitation: 503, Emission: 518
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 57308 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backboneDronpa
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVinculin
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Alt nameNM_003373.3
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SpeciesH. sapiens (human)
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Entrez GeneVCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL, VINC)
- Promoter CMV
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Tag
/ Fusion Protein
- Dronpa
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 503; Emission = 518
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Dronpa-Vinculin-23 was a gift from Michael Davidson (Addgene plasmid # 57308 ; http://n2t.net/addgene:57308 ; RRID:Addgene_57308) -
For your References section:
The fluorescent protein color palette. Olenych SG, Claxton NS, Ottenberg GK, Davidson MW. Curr Protoc Cell Biol. 2007 Sep;Chapter 21:Unit 21.5. doi: 10.1002/0471143030.cb2105s36. 10.1002/0471143030.cb2105s36 PubMed 18228502