Dronpa-Mito-7
(Plasmid
#57288)
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PurposeLocalization: Mitochondria, Excitation: 503, Emission: 518
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 57288 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backboneDronpa
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMito
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Alt nameNM_004074.2
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SpeciesH. sapiens (human)
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Entrez GeneCOX8A (a.k.a. COX, COX8, COX8-2, COX8L, MC4DN15, VIII, VIII-L)
- Promoter CMV
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Tag
/ Fusion Protein
- Dronpa
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 503; Emission = 518
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Dronpa-Mito-7 was a gift from Michael Davidson (Addgene plasmid # 57288 ; http://n2t.net/addgene:57288 ; RRID:Addgene_57288) -
For your References section:
Photoconversion in orange and red fluorescent proteins. Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW. Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009 Apr 12. 10.1038/nmeth.1319 PubMed 19363494