PA-mCherry1-Vimentin-7
(Plasmid
#57202)
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PurposeLocalization: Intermediate Filaments, Excitation: 400 / 504, Emission: 515 / 517
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 57202 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePA-mCherry1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVimentin
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Alt nameNM_003380.3
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SpeciesH. sapiens (human)
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Entrez GeneVIM
- Promoter CMV
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Tag
/ Fusion Protein
- PA-mCherry1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 400 / 504; Emission = 515 / 517
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PA-mCherry1-Vimentin-7 was a gift from Michael Davidson (Addgene plasmid # 57202 ; http://n2t.net/addgene:57202 ; RRID:Addgene_57202) -
For your References section:
Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes. York AG, Ghitani A, Vaziri A, Davidson MW, Shroff H. Nat Methods. 2011 Apr;8(4):327-33. doi: 10.1038/nmeth.1571. Epub 2011 Feb 13. 10.1038/nmeth.1571 PubMed 21317909