mPA-GFP-Paxillin-22
(Plasmid
#57153)
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PurposeLocalization: Focal Adhesions, Excitation: 400 / 504, Emission: 515 / 517
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 57153 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemPA-GFP
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePaxillin
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Alt nameNM_204984.1
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SpeciesG. gallus (chicken)
- Promoter CMV
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Tag
/ Fusion Protein
- mPA-GFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 400 / 504; Emission = 515 / 517
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mPA-GFP-Paxillin-22 was a gift from Michael Davidson (Addgene plasmid # 57153 ; http://n2t.net/addgene:57153 ; RRID:Addgene_57153) -
For your References section:
Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes. Shroff H, Galbraith CG, Galbraith JA, White H, Gillette J, Olenych S, Davidson MW, Betzig E. Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 Dec 12. 10.1073/pnas.0710517105 PubMed 18077327