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Addgene

pTH
(Plasmid #56659)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 56659 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 4361
  • Total vector size (bp) 6817
  • Modifications to backbone
    Dual promoter/terminator
  • Vector type
    Synthetic Biology ; Diatom Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KanR
  • Species
    E. coli
  • Insert Size (bp)
    970

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: There are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. They should not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH was a gift from Philip Weyman (Addgene plasmid # 56659 ; http://n2t.net/addgene:56659 ; RRID:Addgene_56659)

  • For your References section:

    Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis. Weyman PD, Beeri K, Lefebvre SC, Rivera J, McCarthy JK, Heuberger AL, Peers G, Allen AE, Dupont CL. Plant Biotechnol J. 2014 Oct 10. doi: 10.1111/pbi.12254. 10.1111/pbi.12254 PubMed 25302562
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