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pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1
(Plasmid #56658)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 56658 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6.3/V5-DEST
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 9387
  • Total vector size (bp) 10900
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human PKCi
  • Alt name
    protein kinase C iota
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1512
  • Mutation
    A120E and deletion of PB1 domain (aa 24–98)
  • GenBank ID
    NM_002740.4 NP_002731.3
  • Entrez Gene
    PRKCI (a.k.a. DXS1179E, PKCI, nPKC-iota)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5/His (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CMV forward primer
  • 3′ sequencing primer V5(C–term) reverse primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1 was a gift from Pedro Salas (Addgene plasmid # 56658 ; http://n2t.net/addgene:56658 ; RRID:Addgene_56658)

  • For your References section:

    Par-complex aPKC and Par3 cross-talk with innate immunity NF-kappaB pathway in epithelial cells. Forteza R, Wald FA, Mashukova A, Kozhekbaeva Z, Salas PJ. Biol Open. 2013 Oct 8;2(11):1264-9. doi: 10.1242/bio.20135918. eCollection 2013. BIO20135918 [pii] PubMed 24244864
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