pEGFP-C1-M3(T3D)471-721
(Plasmid #56657)

• Purpose: GFP reporter for cytoplasmic inclusions formed by the fused, C-terminal region of protein muNS from mammalian orthoreovirus Type 3 Dearing
• Depositing Lab: Max Nibert
• Publication: Broering et al J Virol. 2005 May;79(10):6194-206.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: BD Biosciences
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 5484
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: M3(T3D)421-721
• Species: mammalian orthoreovirus
• Insert Size (bp): 753
• Mutation: insert encodes aa 471-721 + native stop codon of GenBank AF174384.1
• GenBank ID: AF174384.1
• Promoter: CMV-IE
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: EGFP_C
• 3′ sequencing primer: EBV_rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry