YPet-EB3-7
(Plasmid
#56634)
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PurposeLocalization: MT End Binding Protein, Excitation: 513, Emission: 527
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 56634 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backboneYPet
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEB3
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Alt nameNM_012326.2
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SpeciesH. sapiens (human)
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Entrez GeneMAPRE3 (a.k.a. EB3, EBF3, EBF3-S, RP3)
- Promoter CMV
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Tag
/ Fusion Protein
- YPet (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 513; Emission = 527
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YPet-EB3-7 was a gift from Michael Davidson (Addgene plasmid # 56634 ; http://n2t.net/addgene:56634 ; RRID:Addgene_56634) -
For your References section:
Advances in fluorescent protein technology. Shaner NC, Patterson GH, Davidson MW. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60 10.1242/jcs.005801 PubMed 18057027