EYFP-Mito-7
(Plasmid #56596)

• Purpose: Localization: Mitochondria, Excitation: 513, Emission: 527
• Depositing Lab: Michael Davidson
• Publication: Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 10.1101/pdb.top63.

Backbone

• Vector backbone: EYFP
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mito
• Alt name: NM_004074.2
• Species: H. sapiens (human)
• Entrez Gene: COX8A (a.k.a. COX, COX8, COX8-2, COX8L, MC4DN15, VIII, VIII-L)
• Promoter: CMV
• Tag / Fusion Protein:
  • EYFP

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • EYFP-Mito-7 Data Supplement
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

. Excitation = 513; Emission = 527