EGFP-Tubulin-6
(Plasmid #56450)

• Purpose: Localization: Microtubules, Excitation: 488, Emission: 507
• Depositing Lab: Michael Davidson
• Publication: Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 10.1101/pdb.top63.

Backbone

• Vector backbone: EGFP
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tubulin
• Alt name: NM_006082.2
• Species: H. sapiens (human)
• Entrez Gene: TUBA1B (a.k.a. K-ALPHA-1)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • EGFP-Tubulin-6 Data Supplement
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

. Excitation = 488; Emission = 507