EGFP-Rab11a-7
(Plasmid #56444)

• Purpose: Localization: Ras GTPase, Excitation: 488, Emission: 507
• Depositing Lab: Michael Davidson
• Publication: Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.1111/j.1600-0854.2010.01062.x. Epub 2010 Mar 17.

Backbone

• Vector backbone: EGFP
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rab11a
• Alt name: NM_004663.3
• Species: H. sapiens (human)
• Entrez Gene: RAB11A (a.k.a. YL8)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • EGFP-Rab11a-7 Data Supplement
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

. Excitation = 488; Emission = 507