EGFP-Rab7a-7
(Plasmid
#56443)
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PurposeLocalization: Ras GTPase, Excitation: 488, Emission: 507
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 56443 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backboneEGFP
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRab7a
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Alt nameNM_004637.5
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SpeciesH. sapiens (human)
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MutationN117S in NM_004637.5
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Entrez GeneRAB7A (a.k.a. CMT2B, PRO2706, RAB7)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 488; Emission = 507
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-Rab7a-7 was a gift from Michael Davidson (Addgene plasmid # 56443 ; http://n2t.net/addgene:56443 ; RRID:Addgene_56443) -
For your References section:
The membrane-associated protein, supervillin, accelerates F-actin-dependent rapid integrin recycling and cell motility. Fang Z, Takizawa N, Wilson KA, Smith TC, Delprato A, Davidson MW, Lambright DG, Luna EJ. Traffic. 2010 Jun;11(6):782-99. doi: 10.1111/j.1600-0854.2010.01062.x. Epub 2010 Mar 17. 10.1111/j.1600-0854.2010.01062.x PubMed 20331534