mAzami Green-Vinculin-23
(Plasmid
#56406)
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PurposeLocalization: Focal Adhesions, Excitation: 492, Emission: 505
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 56406 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemAzami Green
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVinculin
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Alt nameNM_003373.3
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SpeciesH. sapiens (human)
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Entrez GeneVCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL, VINC)
- Promoter CMV
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Tag
/ Fusion Protein
- mAzami Green (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 492; Emission = 505
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mAzami Green-Vinculin-23 was a gift from Michael Davidson (Addgene plasmid # 56406 ; http://n2t.net/addgene:56406 ; RRID:Addgene_56406)