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Addgene

mVenus-CENPB-N-22
(Plasmid #56325)

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Item Catalog # Description Quantity Price (USD)
Plasmid 56325 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    mVenus-N1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CENPB
  • Alt name
    NM_001810.5
  • Species
    H. sapiens (human)
  • Mutation
    aa 1-169 of CENPB (DNA Binding Domain)
  • Entrez Gene
    CENPB
  • Promoter CMV
  • Tag / Fusion Protein
    • mVenus (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 515; Emission = 528

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mVenus-CENPB-N-22 was a gift from Michael Davidson (Addgene plasmid # 56325 ; http://n2t.net/addgene:56325 ; RRID:Addgene_56325)

  • For your References section:

    Fluorescent protein tracking and detection: fluorescent protein structure and color variants. Rizzo MA, Davidson MW, Piston DW. Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 10.1101/pdb.top63. 10.1101/pdb.top63 PubMed 20150100
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