mIFP12-LASP1-C-10
(Plasmid
#56257)
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PurposeLocalization: Focal Adhesions, Excitation: 683, Emission: 704
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 56257 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemIFP12-C1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLASP1
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Alt nameNM_006148
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SpeciesH. sapiens (human)
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MutationA209V
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Entrez GeneLASP1 (a.k.a. Lasp-1, MLN50)
- Promoter CMV
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Tag
/ Fusion Protein
- mIFP12 (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 683; Emission = 704
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mIFP12-LASP1-C-10 was a gift from Michael Davidson (Addgene plasmid # 56257 ; http://n2t.net/addgene:56257 ; RRID:Addgene_56257) -
For your References section:
A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K, Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, Davidson MW, Shu X. Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 2015 Jun 22. 10.1038/nmeth.3447 PubMed 26098020
