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PurposeLocalization: Lysosome Membrane, Excitation: 604, Emission: 659
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 56166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemCardinal
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLAMP1
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Alt nameNM_012857.2
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SpeciesR. norvegicus (rat)
- Promoter CMV
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Tag
/ Fusion Protein
- mCardinal
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 604; Emission = 659
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCardinal-Lysosomes-20 was a gift from Michael Davidson (Addgene plasmid # 56166 ; http://n2t.net/addgene:56166 ; RRID:Addgene_56166) -
For your References section:
Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein. Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia KC, Contag CH, Shen K, Blau HM, Lin MZ. Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888. 10.1038/nmeth.2888 PubMed 24633408