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Addgene

mCardinal-Fibrillarin-7
(Plasmid #56160)

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Item Catalog # Description Quantity Price (USD)
Plasmid 56160 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    mCardinal
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fibrillarin
  • Alt name
    NM_001436.3
  • Species
    H. sapiens (human)
  • Mutation
    R36Q, S66F
  • Entrez Gene
    FBL (a.k.a. FIB, FLRN, Nop1, RNU3IP1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCardinal

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 604; Emission = 659

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCardinal-Fibrillarin-7 was a gift from Michael Davidson (Addgene plasmid # 56160 ; http://n2t.net/addgene:56160 ; RRID:Addgene_56160)

  • For your References section:

    Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein. Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia KC, Contag CH, Shen K, Blau HM, Lin MZ. Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888. 10.1038/nmeth.2888 PubMed 24633408
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