FusionRed-Fascin-C-10
(Plasmid #56117)

• Purpose: Localization: Cytoskeleton, Excitation: 580, Emission: 608
• Depositing Lab: Michael Davidson
• Publication: Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.

Backbone

• Vector backbone: FusionRed-C1
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fascin
• Alt name: NM_003088.2
• Species: H. sapiens (human)
• Entrez Gene: FSCN1 (a.k.a. FAN1, HSN, SNL, p55)
• Promoter: CMV
• Tag / Fusion Protein:
  • FusionRed (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • FusionRed-Fascin-C-10 Data Supplement
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Industry MTA
  • Evrogen Ancillary Agreement for FP Distribution to Industry

Depositor Comments

mKate2.7C = FusionRed. Excitation = 580; Emission = 608