mKate1.31-CytERM-N-17
(Plasmid
#56080)
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PurposeLocalization: Endoplasmic Reticulum, Excitation: 588, Emission: 635
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 56080 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemKate1.31-N1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCytERM
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Alt nameCytochrome p450
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Alt nameNM_001171120.1
- Promoter CMV
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Tag
/ Fusion Protein
- mKate1.31 (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cytochrome p450 mKate1.3 = mKate2. Excitation = 588; Emission = 635
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mKate1.31-CytERM-N-17 was a gift from Michael Davidson (Addgene plasmid # 56080 ; http://n2t.net/addgene:56080 ; RRID:Addgene_56080) -
For your References section:
Far-red fluorescent tags for protein imaging in living tissues. Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM. Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949. 10.1042/BJ20081949 PubMed 19143658
