mKate-H1-10
(Plasmid #56057)

• Purpose: Localization: Nucleus/Histones, Excitation: 588, Emission: 635
• Depositing Lab: Michael Davidson
• Publication: Davidson Red fluorescent proteins (unpublished)

Backbone

• Vector backbone: mKate
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H1
• Alt name: Mouse Histone H1
• Species: M. musculus (mouse)
• Entrez Gene: H1f0 (a.k.a. D130017D06Rik, H1(0), H1-0, H1fv)
• Promoter: CMV
• Tag / Fusion Protein:
  • mKate

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mKate-H1-10 Data Supplement

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Industry MTA
  • Evrogen Ancillary Agreement for FP Distribution to Industry

Depositor Comments

. Excitation = 588; Emission = 635