mPlum-H2B-6
(Plasmid
#55976)
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PurposeLocalization: Nucleus/Histones, Excitation: 590, Emission: 649
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 55976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemPlum
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH2B
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Alt nameHuman Histone H2B
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Alt nameNM_021058.3
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SpeciesH. sapiens (human)
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MutationD26G and V119I in H2B
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Entrez GeneH2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
- Promoter CMV
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Tag
/ Fusion Protein
- mPlum
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
H. sapiens (human) Histone H2B. Excitation = 590; Emission = 649
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mPlum-H2B-6 was a gift from Michael Davidson (Addgene plasmid # 55976 ; http://n2t.net/addgene:55976 ; RRID:Addgene_55976) -
For your References section:
The fluorescent protein color palette. Olenych SG, Claxton NS, Ottenberg GK, Davidson MW. Curr Protoc Cell Biol. 2007 Sep;Chapter 21:Unit 21.5. doi: 10.1002/0471143030.cb2105s36. 10.1002/0471143030.cb2105s36 PubMed 18228502