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Addgene

JRed-Alpha-Actinin-19
(Plasmid #55927)

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Item Catalog # Description Quantity Price (USD)
Plasmid 55927 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    JRed
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Alpha-Actinin
  • Alt name
    NM_001102.3
  • Species
    H. sapiens (human)
  • Mutation
    T9P
  • Entrez Gene
    ACTN1 (a.k.a. BDPLT15)
  • Promoter CMV
  • Tag / Fusion Protein
    • JRed (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 584; Emission = 610

There's an EGFP directly downstream of JRed however it is not in frame with Alpha Actinin or JRed.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JRed-Alpha-Actinin-19 was a gift from Michael Davidson (Addgene plasmid # 55927 ; http://n2t.net/addgene:55927 ; RRID:Addgene_55927)
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