mRFP1-Cx43-7
(Plasmid
#55919)
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PurposeLocalization: Gap Junctions, Excitation: 584, Emission: 607
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 55919 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemRFP1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCx43
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Alt nameRat Alpha-1 Connexin 43
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Alt nameNM_012567.2
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SpeciesR. norvegicus (rat)
- Promoter CMV
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Tag
/ Fusion Protein
- mRFP1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
R. norvegicus (rat) Alpha-1 Connexin 43. Excitation = 584; Emission = 607
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mRFP1-Cx43-7 was a gift from Michael Davidson (Addgene plasmid # 55919 ; http://n2t.net/addgene:55919 ; RRID:Addgene_55919)