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Addgene

pBAV1k-T5-LUX
(Plasmid #55800)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55800 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBAV1k
  • Backbone size w/o insert (bp) 2709
  • Total vector size (bp) 8599
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The copy number of pBAV1k plasmids are not well controlled, so purification yields vary. A lacI+ E. coli strain will prevent constitutive expression of the lux operon.
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    luxA
  • Species
    Photorhabdus luminescens
  • Insert Size (bp)
    1082
  • Entrez Gene
    luxA (a.k.a. plu2081)
  • Promoter T5

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    luxB
  • Species
    Photorhabdus luminescens
  • Insert Size (bp)
    983

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    luxC
  • Species
    Photorhabdus luminescens
  • Insert Size (bp)
    1442

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    luxD
  • Species
    Photorhabdus luminescens
  • Insert Size (bp)
    923

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Light production is highly dependent upon growth phase and the availability of oxygen (see http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0088159 ).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAV1k-T5-LUX was a gift from Ichiro Matsumura (Addgene plasmid # 55800)

  • For your References section:

    Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria. Bryksin AV, Matsumura I. PLoS One. 2010 . 5(10):e13244. 10.1371/journal.pone.0013244 PubMed 20949038
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