Skip to main content
Addgene

G protein alpha-s(alpha3beta5/G226A/A366S)
(Plasmid #55797)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55797 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNAI/Amp
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 6387
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha-s (alpha3beta5/G226A/A366S)
  • Alt name
    Gnas
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1586
  • Mutation
    N271K, K274D, R280K, T284D, I285T, G226A, A366S in alpha-s sequence
  • Entrez Gene
    Gnas (a.k.a. ALEX, G-alpha-8, Gnas1, Gnpas, Nesp55, SCG6)
  • Promoter CMV
  • Tag / Fusion Protein
    • internal EE epitope (residues 189-194 in alpha-s sequence are EYMPTE)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G protein alpha-s(alpha3beta5/G226A/A366S) was a gift from Catherine Berlot (Addgene plasmid # 55797 ; http://n2t.net/addgene:55797 ; RRID:Addgene_55797)

  • For your References section:

    A highly effective dominant negative alpha s construct containing mutations that affect distinct functions inhibits multiple Gs-coupled receptor signaling pathways. Berlot CH. J Biol Chem. 2002 Jun 7;277(23):21080-5. Epub 2002 Apr 1. 10.1074/jbc.M201330200 PubMed 11927592
Related items:
Commonly requested with: