mito-dendra2
(Plasmid #55796)

• Purpose: photo-activatable mitochondrial marker for expression in mammalian cells
• Depositing Lab: David Chan
• Publication: Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10.1002/dvg.22050. Epub 2012 Aug 11.

Backbone

• Vector backbone: pCMV
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 3920
• Total vector size (bp): 4740
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mito-Dendra2
• Alt name: cox8a-Dendra2
• Species: H. sapiens (human)
• Insert Size (bp): 816
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dendra2 is from Evrogen.
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry