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PurposeDominant negative Dynamin 1 mutant tagged with mRFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 55795 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneDerived from pEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3950
- Total vector size (bp) 7286
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Modifications to backboneGFP was removed as a BamHI/NotI cassette and replaced with mRFP.
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDynamin-1(K44A)-mRFP
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Alt nameDNM1
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SpeciesR. norvegicus (rat); Discosoma sp.
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Insert Size (bp)3336
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MutationDynamin-1(K44A)-mRFP was produced from Dynamin-1(K44A)-GFP by replacing GFP with mRFP.
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GenBank ID
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Entrez GeneDnm1 (a.k.a. Dnm)
- Promoter CMV
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Tag
/ Fusion Protein
- mRFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site None (unknown if destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDyn1(K44A)-GFP was obtained from Pietro De Camilli, Howard Hughes Medical Institute, Yale University, New Haven, CT. mRFP was obtained from Roger Tsien, University of California, San Diego, California.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Dyn1(K44A)-mRFP was a gift from Catherine Berlot (Addgene plasmid # 55795 ; http://n2t.net/addgene:55795 ; RRID:Addgene_55795) -
For your References section:
Live cell imaging of Gs and the beta2-adrenergic receptor demonstrates that both alphas and beta1gamma7 internalize upon stimulation and exhibit similar trafficking patterns that differ from that of the beta2-adrenergic receptor. Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH. J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5. 10.1074/jbc.M405151200 PubMed 15297467