G protein alpha-o-R179C-EE
(Plasmid #55780)

• Purpose: This is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.
• Depositing Lab: Catherine Berlot
• Publication: Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.

Backbone

• Vector backbone: pcDNAI/Amp
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4800
• Total vector size (bp): 6182
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G protein alpha-o with constitutively activating R179C mutation
• Alt name: Gnao
• Species: R. norvegicus (rat)
• Insert Size (bp): 1382
• Mutation: Arginine 179 was replaced with cysteine
• Entrez Gene: Gnao1 (a.k.a. Gnao, Hg1g, RATBPGTPC)
• Promoter: CMV
• Tag / Fusion Protein:
  • EE epitope was introduced internally with D167E and Q169M mutations.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry