mCherry-Mem
(Plasmid #55779)

• Purpose: This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.
• Depositing Lab: Catherine Berlot
• Publication: Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.

Backbone

• Vector backbone: Derived from pEYFP-Mem
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3942
• Total vector size (bp): 4783
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry-Mem
• Species: Discosoma sp.
• Insert Size (bp): 841
• Promoter: CMV
• Tag / Fusion Protein:
  • The N-terminal 20 residues of neuromodulin are fused to the N-terminus of mCherry.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: sV40pA-Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mCherry was obtained from Roger Tsien, University of California, San Diego, CA. pEYFP-Mem was purchased from Clontech.
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Reference for mCherry:
Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004;22(12):1567-72. PubMed PMID: 15558047.

The K196R mutation in mCherry has no functional consequence.