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Addgene

msEGFP-PACR_pcDNA3
(Plasmid #55774)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55774 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 1700
  • Total vector size (bp) 7100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PACR
  • Species
    Synthetic
  • Insert Size (bp)
    1700
  • Promoter CMV
  • Tag / Fusion Protein
    • msEGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    msEGFP-PACR_pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 55774 ; http://n2t.net/addgene:55774 ; RRID:Addgene_55774)

  • For your References section:

    Optical control of the ca(2+) concentration in a live specimen with a genetically encoded ca(2+)-releasing molecular tool. Fukuda N, Matsuda T, Nagai T. ACS Chem Biol. 2014 May 16;9(5):1197-203. doi: 10.1021/cb400849n. Epub 2014 Mar 24. 10.1021/cb400849n PubMed 24625002
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