Skip to main content
Addgene

BglB in pET29b(+)
(Plasmid #55773)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55773 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET29b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5232
  • Total vector size (bp) 6607
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Beta-glucosidase B
  • Species
    Synthetic
  • Insert Size (bp)
    1375
  • Entrez Gene
    bglB (a.k.a. PPM_0898)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BglB in pET29b(+) was a gift from Justin Siegel (Addgene plasmid # 55773 ; http://n2t.net/addgene:55773 ; RRID:Addgene_55773)

  • For your References section:

    Kinetic Characterization of 100 Glycoside Hydrolase Mutants Enables the Discovery of Structural Features Correlated with Kinetic Constants. Carlin DA, Caster RW, Wang X, Betzenderfer SA, Chen CX, Duong VM, Ryklansky CV, Alpekin A, Beaumont N, Kapoor H, Kim N, Mohabbot H, Pang B, Teel R, Whithaus L, Tagkopoulos I, Siegel JB. PLoS One. 2016 Jan 27;11(1):e0147596. doi: 10.1371/journal.pone.0147596. eCollection 2016. 10.1371/journal.pone.0147596 PubMed 26815142
Related items:
Commonly requested with: