pBacPAK p110b WT
(Plasmid
#55724)
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PurposeHis3 GST tagged WT p110 beta
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 55724 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBacPAK
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5500
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep110 beta
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Alt namePI3K
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Alt namep110b
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SpeciesM. musculus (mouse)
- Promoter polyhedrin promoter
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Tag
/ Fusion Protein
- His3 GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer Polyhedrin forward (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
murine p110α and p110β constructs (wild type and RBD DM) subcloned into pBacPAK-His3-GST (from protein purification facility). So these constructs encode GST-tagged p110, which can be cleaved of using rhinovirus 3C protease
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBacPAK p110b WT was a gift from Julian Downward (Addgene plasmid # 55724 ; http://n2t.net/addgene:55724 ; RRID:Addgene_55724) -
For your References section:
RAS and RHO families of GTPases directly regulate distinct phosphoinositide 3-kinase isoforms. Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, Diefenbacher M, Stamp G, Downward J. Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031. 10.1016/j.cell.2013.04.031 PubMed 23706742