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Addgene

pSG5-Myr-FLAG-p110α-DM
(Plasmid #55713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p110 alpha
  • Alt name
    PI3K
  • Species
    M. musculus (mouse)
  • Mutation
    T207D, K227A
  • Entrez Gene
    Pik3ca (a.k.a. 6330412C24Rik, caPI3K, p110, p110alpha)
  • Promoter T7
  • Tag / Fusion Protein
    • myristoylation tag (Myr-FLAG) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-Myr-FLAG-p110α-DM was a gift from Julian Downward (Addgene plasmid # 55713 ; http://n2t.net/addgene:55713 ; RRID:Addgene_55713)

  • For your References section:

    RAS and RHO families of GTPases directly regulate distinct phosphoinositide 3-kinase isoforms. Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, Diefenbacher M, Stamp G, Downward J. Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031. 10.1016/j.cell.2013.04.031 PubMed 23706742
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