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Addgene

pcDNATM3.1/V5-His TOPO-PKCi-A120E
(Plasmid #55687)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55687 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/V5-His TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5523
  • Total vector size (bp) 7313
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Homo sapiens protein kinase C, iota (PRKCI)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1790
  • Mutation
    A120E
  • GenBank ID
    NM_002740.4 NP_002731.3
  • Entrez Gene
    PRKCI (a.k.a. DXS1179E, PKCI, nPKC-iota)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5/His (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNATM3.1/V5-His TOPO-PKCi-A120E was a gift from Pedro Salas (Addgene plasmid # 55687 ; http://n2t.net/addgene:55687 ; RRID:Addgene_55687)

  • For your References section:

    Atypical protein kinase C (iota) activates ezrin in the apical domain of intestinal epithelial cells. Wald FA, Oriolo AS, Mashukova A, Fregien NL, Langshaw AH, Salas PJ. J Cell Sci. 2008 Mar 1;121(Pt 5):644-54. doi: 10.1242/jcs.016246. Epub 2008 Feb 12. 10.1242/jcs.016246 PubMed 18270268
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