Skip to main content
Addgene

RALA
(Plasmid #55657)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55657 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEX2T
  • Backbone manufacturer
    GE Healthcare Life Sciences
  • Backbone size w/o insert (bp) 4948
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21 codon plus ril de3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RALA
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Rala (a.k.a. 3010001O15Rik, AW322615, Ral, Rasl1)
  • Promoter lac
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • Thrombin cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site MfeI (destroyed during cloning)
  • 5′ sequencing primer pGEX5
  • 3′ sequencing primer pGEX3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RALA was a gift from Julian Downward (Addgene plasmid # 55657 ; http://n2t.net/addgene:55657 ; RRID:Addgene_55657)

  • For your References section:

    RAS and RHO families of GTPases directly regulate distinct phosphoinositide 3-kinase isoforms. Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, Diefenbacher M, Stamp G, Downward J. Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031. 10.1016/j.cell.2013.04.031 PubMed 23706742
Related items:
Commonly requested with: