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Addgene

pAAV-Ef1a-DIO SwiChRca-TS-EYFP
(Plasmid #55631)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55631 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Backbone size w/o insert (bp) 5600
  • Total vector size (bp) 7317
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iC1C2-TS-EYFP
  • Alt name
    SwiChRca
  • Species
    Synthetic
  • Insert Size (bp)
    1713
  • Mutation
    C128A
  • Promoter Ef1a
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer CACCCACACAAAGGAAAAGGGCC
  • 3′ sequencing primer GCAATAGCATGATACAAAGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Ef1a-DIO SwiChRca-TS-EYFP was a gift from Karl Deisseroth (Addgene plasmid # 55631 ; http://n2t.net/addgene:55631 ; RRID:Addgene_55631)

  • For your References section:

    Structure-guided transformation of channelrhodopsin into a light-activated chloride channel. Berndt A, Lee SY, Ramakrishnan C, Deisseroth K. Science. 2014 Apr 25;344(6182):420-4. doi: 10.1126/science.1252367. 10.1126/science.1252367 PubMed 24763591
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