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pLgw-MBD3-V5-Dam
(Plasmid #55605)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55605 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLgw-RFC1-V5-EcoDam
  • Backbone manufacturer
    Dr. Bas van Steensel, Netherlands Cancer Institute
  • Backbone size w/o insert (bp) 10208
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MBD3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    780
  • GenBank ID
    BC043619
  • Entrez Gene
    MBD3
  • Promoter HSP
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • DamID (DNA adenine methyltransferase identification) (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human MBD3 sequence was obtained from Thermo Scientific cDNA clone MGC:50958 IMAGE:6156267

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLgw-MBD3-V5-Dam was a gift from Paul Wade (Addgene plasmid # 55605 ; http://n2t.net/addgene:55605 ; RRID:Addgene_55605)

  • For your References section:

    MBD3 localizes at promoters, gene bodies and enhancers of active genes. Shimbo T, Du Y, Grimm SA, Dhasarathy A, Mav D, Shah RR, Shi H, Wade PA. PLoS Genet. 2013 Dec;9(12):e1004028. doi: 10.1371/journal.pgen.1004028. Epub 2013 Dec 26. PGENETICS-D-13-01724 [pii] PubMed 24385926
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