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Purpose(Empty Backbone) transgene expression driven by ALPHA MHC promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 55594 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript II SK+
- Backbone size (bp) 2900
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Vector typeMammalian Expression
- Promoter alpha MHC
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The distance between the two Not1 sites is ~6kb
Clone into the Sal1 site (or Sal/Hind3 site).
Release the pBluescript backbone with Not1 for transgenics.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJG/ALPHA MHC was a gift from Jeffrey Robbins (Addgene plasmid # 55594 ; http://n2t.net/addgene:55594 ; RRID:Addgene_55594) -
For your References section:
Isolation and characterization of the mouse cardiac myosin heavy chain genes. Gulick J, Subramaniam A, Neumann J, Robbins J. J Biol Chem. 1991 May 15;266(14):9180-5. PubMed 2026617
Map uploaded by the depositor.
