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Addgene

mTFP1-Vimentin-7
(Plasmid #55515)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55515 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mTFP1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vimentin
  • Alt name
    NM_003380.3
  • Species
    H. sapiens (human)
  • Entrez Gene
    VIM
  • Promoter CMV
  • Tag / Fusion Protein
    • mTFP1 (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 462; Emission = 492

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mTFP1-Vimentin-7 was a gift from Michael Davidson (Addgene plasmid # 55515 ; http://n2t.net/addgene:55515 ; RRID:Addgene_55515)

  • For your References section:

    Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging. Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE. BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13. 10.1186/1741-7007-6-13 PubMed 18325109
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