mTFP1-Golgi-7
(Plasmid
#55486)
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PurposeLocalization: GalT Targeting Sequence, Excitation: 462, Emission: 492
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 55486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemTFP1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGolgi
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Alt nameNM_001497.3
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SpeciesH. sapiens (human)
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Mutationaa 1-82 of NM_001497.3
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Entrez GeneB4GALT1 (a.k.a. B4GAL-T1, CDG2D, CLDLFIB, GGTB2, GT1, GTB, beta4Gal-T1)
- Promoter CMV
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Tag
/ Fusion Protein
- mTFP1 (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 462; Emission = 492
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mTFP1-Golgi-7 was a gift from Michael Davidson (Addgene plasmid # 55486 ; http://n2t.net/addgene:55486 ; RRID:Addgene_55486) -
For your References section:
Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging. Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE. BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13. 10.1186/1741-7007-6-13 PubMed 18325109