mTFP1-Farnesyl-5
(Plasmid #55481)

• Purpose: Localization: Plasma Membrane, Excitation: 462, Emission: 492
• Depositing Lab: Michael Davidson
• Publication: Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.

Backbone

• Vector backbone: mTFP1
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Farnesyl
• Alt name: NM_001130442.1
• Species: H. sapiens (human)
• Mutation: encodes final 20 aa of NM_001130442.1
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
• Promoter: CMV
• Tag / Fusion Protein:
  • mTFP1 (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mTFP1-Farnesyl-5 Data Supplement

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Encodes mTFP1 with the C-terminal farnesylation signal from c-Ras. Excitation = 462; Emission = 492