mCerulean3-Lysosomes-20
(Plasmid
#55428)
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PurposeLocalization: Lysosome Membrane, Excitation: 433, Emission: 475
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 55428 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemCerulean3
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLAMP1
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Alt nameNM_012857.2
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SpeciesR. norvegicus (rat)
- Promoter CMV
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Tag
/ Fusion Protein
- mCerulean3 (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 433; Emission = 475
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCerulean3-Lysosomes-20 was a gift from Michael Davidson (Addgene plasmid # 55428 ; http://n2t.net/addgene:55428 ; RRID:Addgene_55428) -
For your References section:
An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching. Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, Day RN, Wachter RM, Davidson MW, Rizzo MA. PLoS One. 2011 . 6(3):e17896. 10.1371/journal.pone.0017896 PubMed 21479270
