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mCerulean3-H2B-C-10
(Plasmid #55422)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55422 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCerulean3-C1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B
  • Alt name
    Human Histone H2B
  • Alt name
    NM_021058.3
  • Species
    H. sapiens (human)
  • Mutation
    D26G and V119I in H2B
  • Entrez Gene
    H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCerulean3 (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

H. sapiens (human) Histone H2B. Excitation = 433; Emission = 475

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCerulean3-H2B-C-10 was a gift from Michael Davidson (Addgene plasmid # 55422 ; http://n2t.net/addgene:55422 ; RRID:Addgene_55422)

  • For your References section:

    An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching. Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, Day RN, Wachter RM, Davidson MW, Rizzo MA. PLoS One. 2011 . 6(3):e17896. 10.1371/journal.pone.0017896 PubMed 21479270
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