ECFP-Annexin-12
(Plasmid
#55340)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 55340 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
* Log in to view industry pricing.
Backbone
-
Vector backboneECFP
- Backbone size w/o insert (bp) 4750
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameAnnexin (A4)
-
Alt nameNM_001153.3
-
SpeciesH. sapiens (human)
-
Entrez GeneANXA4 (a.k.a. ANX4, HEL-S-274, P32.5, PAP-II, PIG28, PP4-X, ZAP36)
- Promoter CMV
-
Tag
/ Fusion Protein
- ECFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Excitation = 433; Emission = 475. Please note that the Addgene verified CFP sequence differs from the depositor reference sequence, however these mutation (K27R and N164H) do not alter plasmid function.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
ECFP-Annexin-12 was a gift from Michael Davidson (Addgene plasmid # 55340 ; http://n2t.net/addgene:55340 ; RRID:Addgene_55340)
